US 11,806,442 B2
System and methods for harvesting and processing periosteum fibers
Megan Maccagnan, Golden, CO (US); Cameron Mattson, Morrison, CO (US); Cole Watkins, Fort Collins, CO (US); Adrian C. Samaniego, Centennial, CO (US); and William Maslanik, Littleton, CO (US)
Assigned to Allosource, Centennial, CO (US)
Filed by Allosource, Centennial, CO (US)
Filed on Sep. 16, 2019, as Appl. No. 16/571,632.
Claims priority of provisional application 62/732,210, filed on Sep. 17, 2018.
Prior Publication US 2020/0086001 A1, Mar. 19, 2020
Int. Cl. A61L 27/36 (2006.01); C12N 5/071 (2010.01); A61B 17/16 (2006.01)
CPC A61L 27/3608 (2013.01) [A61L 27/3687 (2013.01); A61L 27/3691 (2013.01); C12N 5/0697 (2013.01); A61B 17/1635 (2013.01); A61L 2430/02 (2013.01); A61L 2430/38 (2013.01)] 11 Claims
 
1. A method of harvesting and processing bulk periosteum tissue into a plurality of individual, separated periosteum fibers, the method comprising:
removing, using one or more harvesting tools, a quantity of bulk periosteum tissue from an outer surface of a human long bone;
first washing the quantity of the bulk periosteum tissue in a volume of hydrogen peroxide;
second washing the quantity of the bulk periosteum tissue in a volume of isopropyl alcohol;
third washing the quantity of the bulk periosteum tissue in a volume of phosphate buffered saline (PBS);
compressing the quantity of the bulk periosteum tissue;
cryofracturing the quantity of the bulk periosteum tissue with a given number of cryofracturing pulses until the quantity of the bulk periosteum tissue comprises a plurality of fused periosteum fibers having a desired size based on the given number of cryofracturing pulses; and
using a fiber separator tool to separate the plurality of the fused periosteum fibers from one another into the plurality of individual, separated periosteum fibers, wherein the given number of cryofracturing pulses provides an initial sizing of the fused periosteum prior to the step of using a separating tool to separate the fused periosteum fibers having the desired size based on the given number of cryofracturing pulses.