CPC A61K 35/747 (2013.01) [A61K 35/741 (2013.01); A61K 47/02 (2013.01); A61K 47/10 (2013.01); A61K 47/20 (2013.01)] | 20 Claims |
1. A method for a growth of Akkermansia sp. comprising performing a plurality of inoculation rounds with an increasing amount of growth media,
wherein an inoculation round of the plurality of inoculation rounds comprises use of at least about 5% by volume of a total batch material of a preceding inoculation round of the inoculation round of the plurality of inoculation rounds, and
wherein a growth media of at least one inoculation round of the plurality of inoculation rounds is at least about 20 liters (L).
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