US 11,806,368 B2
Stem cell subpopulations with differential GSTT1 expression or genotype
Lawrence Stanton, Singapore (SG); Padmapriya Sathiyanathan, Singapore (SG); Simon Cool, Singapore (SG); Victor Nurcombe, Singapore (SG); and Rebekah M. Samsonraj, Singapore (SG)
Assigned to Agency for Science, Technology and Research, Singapore (SG)
Filed by Agency for Science, Technology and Research, Singapore (SG)
Filed on May 15, 2020, as Appl. No. 16/875,486.
Application 16/875,486 is a division of application No. 15/318,909, abandoned, previously published as PCT/SG2015/050184, filed on Jun. 26, 2015.
Claims priority of application No. 10201403640X (SG), filed on Jun. 26, 2014.
Prior Publication US 2020/0345782 A1, Nov. 5, 2020
Int. Cl. A61K 35/28 (2015.01); C12Q 1/6881 (2018.01); A61K 35/545 (2015.01); C12N 5/0775 (2010.01)
CPC A61K 35/28 (2013.01) [A61K 35/545 (2013.01); C12N 5/0662 (2013.01); C12N 5/0663 (2013.01); C12Q 1/6881 (2013.01); C12Y 205/01018 (2013.01); C12Q 2600/158 (2013.01)] 5 Claims
 
1. A method for selecting a stem cell donor, the method comprising:
(a) determining the genotype for GSTT1 in a DNA-containing sample isolated from an individual, and selecting as the stem cell donor an individual determined to have a GSTT1 genotype which is known, and/or which would be predicted, to result in decreased expression of GSTT1 relative to individuals homozygous for wildtype; or
(b) determining the level of GSTT1 expression by a stem cell or plurality of stem cells in a sample isolated from an individual, and comparing the determined level of GSTT1 expression to a reference value for the level of GSTT1 expression for that stem cell type, and selecting as the stem cell donor an individual determined to have a stem cell or plurality of stem cells having decreased GSTT1 expression relative to the reference value,
the method further comprising separating and/or isolating a stem cell or plurality of stem cells from the individual selected as a stem cell donor, wherein the stem cell or plurality of stem cells separated and/or isolated from the individual selected as the stem cell donor is a mesenchymal stem cell (MSC) or plurality of MSCs obtained from the bone marrow of the individual selected as a stem cell donor.