	US 11,805,792 B2
	Animal feed with engineered phytases
R. Michael Raab, Arlington, MA (US); Gabor Lazar, Belmont, MA (US); and Binzhang Shen, Boston, MA (US)
Assigned to AGRIVIDA, INC., Woburn, MA (US)
Filed by AGRIVIDA, INC., Woburn, MA (US)
Filed on Dec. 14, 2021, as Appl. No. 17/550,300.
Application 17/550,300 is a continuation of application No. 16/867,928, filed on May 6, 2020, granted, now 11,241,025.
Application 16/867,928 is a continuation of application No. 15/756,231, granted, now 10,687,542, previously published as PCT/US2016/052147, filed on Sep. 16, 2016.
Claims priority of provisional application 62/220,688, filed on Sep. 18, 2015.
Prior Publication US 2022/0095647 A1, Mar. 31, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A23K 10/14 (2016.01); C07K 14/245 (2006.01); C07K 14/38 (2006.01); C07K 14/39 (2006.01); C12N 9/16 (2006.01); C12N 15/80 (2006.01); A23K 20/189 (2016.01); C12N 15/82 (2006.01); C07K 14/00 (2006.01)

CPC A23K 10/14 (2016.05) [A23K 20/189 (2016.05); C07K 14/00 (2013.01); C07K 14/245 (2013.01); C07K 14/38 (2013.01); C07K 14/39 (2013.01); C12N 9/16 (2013.01); C12N 15/80 (2013.01); C12N 15/8257 (2013.01); C12Y 301/03 (2013.01)]

16 Claims

1. An animal feed comprising an engineered phytase comprising a target phytase, a first binding element and a second binding element, wherein each of the first binding element and the second binding element is fused to the target phytase, the first binding element interacts with the second binding element to cause cyclization of the engineered phytase, and enhance thermal stability of the target phytase, and wherein the first binding element or the second binding element comprises a C or N intein, the target phytase comprises the amino acid sequence of SEQ ID NO: 53 and the engineered phytase has enhanced thermal stability compared to the target phytase.