	US 11,805,777 B2
	Engineered pesticidal proteins and methods of controlling plant pests
Christopher Fleming, Research Triangle Park, NC (US); and Richard Sessler, Research Triangle Park, NC (US)
Assigned to Syngenta Participations AG, Basel (CH)
Filed by Syngenta Participations AG, Basel (CH)
Filed on Sep. 23, 2022, as Appl. No. 17/934,613.
Application 17/934,613 is a division of application No. 16/648,323, granted, now 11,484,030, previously published as PCT/US2018/053687, filed on Oct. 1, 2018.
Claims priority of provisional application 62/566,692, filed on Oct. 2, 2017.
Prior Publication US 2023/0148596 A1, May 18, 2023
Int. Cl. C12N 15/82 (2006.01); C07K 14/195 (2006.01); A01N 37/44 (2006.01); A01H 5/10 (2018.01); A01N 63/50 (2020.01)

CPC A01N 37/44 (2013.01) [A01H 5/10 (2013.01); A01N 63/50 (2020.01); C07K 14/195 (2013.01); C12N 15/8286 (2013.01)]

20 Claims

1. A modified Axmi205 toxin, wherein said modified Axmi205 toxin comprises an amino acid sequence having at least 99% identity to the polypeptide of SEQ ID NO: 1 and comprises a substitution of a serine, leucine, alanine, phenylalanine, aspartic acid or arginine at position C507 in the polypeptide of SEQ ID NO:1, and wherein the substitution results in enhanced digestion of the modified Axmi205 toxin by pepsin as compared with the Axmi205 toxin that does not comprise the substitution.