US 7,615,537 B2
	Methods for treating blood coagulation disorders
Abraham Scaria, Framingham, Mass. (US); and Samuel C. Wadsworth, Shrewsbury, Mass. (US)
Assigned to Genzyme Corporation, Cambridge, Mass. (US)
Filed on Oct. 25, 2001, as Appl. No. 10/57,620.
Claims priority of provisional application 60/307492, filed on Jul. 24, 2001.
Claims priority of provisional application 60/243046, filed on Oct. 25, 2000.
Prior Publication US 2003/0229036 A1, Dec. 11, 2003
Int. Cl. A61K 48/00 (2006.01); C12N 15/63 (2006.01)

U.S. Cl. 514—44  [435/320.1]

49 Claims

1. A method at promoting blood coagulation in an individual having a blood coagulation defect and in need thereof, comprising administering to the individual a blood coagulation enhancing effective amount of a DNA vector encoding a human Factor VII polypeptide that can be converted to Factor VIIa when expressed in said individual, said Factor VII polypeptide comprising an enzymatic cleavage site susceptible to cleavage by furin, wherein said enzymatic cleavage site is located in the area of about amino acid 147 through about 154 of said Factor VII and wherein at least one amino acid mutations have been made in said area to create said enzymatic cleavage site, and, whereby cleavage by furin produces two peptides comprising Factor VII heavy chain and Factor VII light chain molecules.