| US 7,615,381 B2 | ||
| Method and apparatus for detecting estradiol and metabolites thereof using an acoustic device | ||
| Brett P. Masters, Watertown, Mass. (US); Michael Miller, Hollis, N.H. (US); Vishal K. Gulati, London (United Kingdom); Mark Lundstrom, Boston, Mass. (US); Alok Srivastava, San Francisco, Calif. (US); and Wayne U. Wang, Cambridge, Mass. (US) | ||
| Assigned to BioScale, Inc., Cambridge, Mass. (US) | ||
| Filed on May 02, 2006, as Appl. No. 11/417,000. | ||
| Application 11/417000 is a continuation in part of application No. 11/183484, filed on Jul. 18, 2005, granted, now 7,300,631. | ||
| Claims priority of provisional application 60/690592, filed on Jun. 15, 2005. | ||
| Claims priority of provisional application 60/676759, filed on May 02, 2005. | ||
| Prior Publication US 2007/0042441 A1, Feb. 22, 2007 | ||
| Int. Cl. G01N 33/553 (2006.01); B32B 27/00 (2006.01); B32B 27/12 (2006.01) | ||
| U.S. Cl. 436—526 [436/523; 436/525; 436/168; 435/7.1; 435/7.94; 435/287.2; 435/287.3; 422/68.1; 422/81; 422/82.13; 422/100; 422/104] | 15 Claims |
| 1. A method for detecting estradiol in a sample, the method comprising the steps of:
a) introducing a fluid comprising a sample and a plurality of magnetic particles coated with a first capture agent capable
of binding estradiol into a fluid chamber, wherein at least one surface of the fluid chamber comprises a flexural plate wave
device having a second capture agent capable of binding estradiol, wherein the flexural plate wave device has a membrane that
is capable of vibrating, and wherein the second capture agent is bound to the membrane;
b) applying a retractable source of magnetic flux positioned external to the fluid chamber close to the membrane to create
a significant magnetic field gradient at a surface of the membrane to attract magnetic particles in the fluid toward the membrane;
c) monitoring a first signal output by said flexural plate wave device, wherein the first signal output is monitored in the
presence of a magnetic flux;
d) after removing the retractable source of magnetic flux, flowing a solution through the fluid chamber to remove magnetic
particles not bound to the membrane;
e) monitoring a second signal output by said flexural plate wave device, wherein the second signal output is monitored in
the absence of the magnetic flux; and
f) comparing the first and the second signal output to a control signal, thereby detecting estradiol in the sample.
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