| US 7,615,370 B2 | ||
| System having device for preventing air bubbles in a hybridization chamber and corresponding method | ||
| Wolfgang Streit, Hallein (Austria); Gyoergy Wenczel, Seekirchen (Austria); Waltraud Lamprecht, Salzburg (Austria); and Heribert Eglauer, Berchtesgaden (Germany) | ||
| Assigned to Tecan Trading AG, Mannedorf (Switzerland) | ||
| Filed on Sep. 01, 2004, as Appl. No. 10/931,432. | ||
| Claims priority of application No. 1144/04 (CH), filed on Jul. 08, 2004; and application No. 20 2004 012 163 U (DE), filed on Aug. 04, 2004. | ||
| Prior Publication US 2006/0008812 A1, Jan. 12, 2006 | ||
| Int. Cl. C12M 1/34 (2006.01); C12M 3/00 (2006.01) | ||
| U.S. Cl. 435—287.2 [435/287.1; 435/286.7; 435/288.5] | 7 Claims |
| 1. A method for preventing air bubbles in an essentially gap-shaped hybridization chamber of a system for hybridizing nucleic
acid samples, proteins, or tissue sections that are immobilized on slides, the method comprising the steps of:
(a) providing an essentially gap-shaped hybridization chamber between a slide and a cover, the cover being positioned in relation
to the slide in such a way that the hybridization chamber is sealed to surrounding air;
(b) essentially filling the hybridization chamber as provided in step (a) with a liquid;
(c) building-up a chamber pressure in the hybridization chamber with a pressure device of said system; and
(d) keeping the chamber pressure at a pressure of at least 100 mbar above the normal atmospheric pressure existing in the
surrounding air during a hybridization procedure.
|