	US 7,615,365 B2
	α(1,6)-linked glucose oligosaccharide hydrolyzing enzyme polynucleotides and polypeptides and methods of use thereof
Perry G. Caimi, Kennett Square, Pa. (US); Mario W. Chen, Chadds Ford, Pa. (US); Vasantha Nagarajan, Wilmington, Del. (US); Jean-Francois Tomb, Wilmington, Del. (US); Siqun Wang, Wilmington, Del. (US); and Yuying Zhang, New Hope, Pa. (US)
Assigned to E.I. du Pont de Nemours and Company, Wilmington, Del. (US)
Filed on Sep. 10, 2007, as Appl. No. 11/852,834.
Application 11/852834 is a division of application No. 10/648152, filed on Aug. 25, 2003, granted, now 7,268,221.
Claims priority of provisional application 60/405896, filed on Aug. 23, 2002.
Prior Publication US 2009/0068704 A1, Mar. 12, 2009
Int. Cl. C12N 9/00 (2006.01); C12N 9/24 (2006.01); C12N 1/20 (2006.01); C12N 15/00 (2006.01); C07H 21/04 (2006.01)

U.S. Cl. 435—200 [435/183; 435/252.3; 435/320.1; 536/23.2]

25 Claims

1. An isolated nucleic acid molecule encoding an α(1,6)-linked glucose oligosaccharide hydrolyzing polypeptide selected from the group consisting of:

(a) an isolated nucleic acid molecule encoding the amino acid sequence SEQ ID NO:2;

(b) a nucleic acid molecule that hybridizes with (a) under the following stringent hybridization conditions: 0.1×SSC, 0.1% SDS, 65° C. and washed with 2×SSC, 0.1% SDS followed by 0.1×SSC, 0.1% SDS; and