| US 7,615,351 B2 | ||
| Two stage nucleic acid amplification using an amplification oligomer | ||
| Gary McMaster, Ann Arbor, Mich. (US); and Yunqing Ma, Cupertino, Calif. (US) | ||
| Assigned to Panomics, Inc., Fremont, Calif. (US) | ||
| Filed on Nov. 06, 2007, as Appl. No. 11/983,216. | ||
| Claims priority of provisional application 60/872199, filed on Dec. 01, 2006. | ||
| Prior Publication US 2008/0176242 A1, Jul. 24, 2008 | ||
| Int. Cl. C12Q 1/68 (2006.01); C07H 21/04 (2006.01) | ||
| U.S. Cl. 435—6 | 42 Claims |
| 1. A method of detecting one or more nucleic acids of interest, which method comprises:
(a) providing a sample comprising or suspected of comprising one or more nucleic acids of interest;
(b) capturing the one or more nucleic acids of interest on a first solid support;
(c) hybridizing one or more first amplification multimers to each of the one or more nucleic acids of interest;
(d) hybridizing one or more amplification oligomers to the one or more first amplification multimers of step (c);
(e) transferring the one or more amplification oligomers that hybridized in step (d) to a second solid support;
(f) hybridizing one or more second amplification multimers to the one or more amplification oligomers captured on the second
solid support;
(g) hybridizing one or more label probes to the one or more second amplification multimers of step (f); and,
(h) detecting the one or more label probes.
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