US 7,612,186 B2
Compositions and methods for producing recombinant proteins
Tarmo Roosild, Las Vegas, Nev. (US); Jason Greenwald, Zurich (Switzerland); Senyon Choe, Solana Beach, Calif. (US); and Roland Riek, Zurich (Switzerland)
Assigned to The Salk Institute for Biological Studies, La Jolla, Calif. (US)
Filed on Dec. 22, 2005, as Appl. No. 11/317,847.
Claims priority of provisional application 60/639174, filed on Dec. 22, 2004.
Prior Publication US 2006/0211087 A1, Sep. 21, 2006
Int. Cl. C07H 21/00 (2006.01); C12N 1/21 (2006.01); C07K 17/02 (2006.01)
U.S. Cl. 536—23.1  [435/252.1; 424/450] 73 Claims
 
1. An isolated nucleic acid molecule encoding a polypeptide of no more than 125 amino acids in length comprising:
(a) an amino acid sequence as set forth in SEQ ID NO: 2;
(b) an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 2, and which is capable of associating with a membrane;
(c) an amino acid sequence that differs from SEQ ID NO: 2 by no more than about 10 conserved amino acid substitutions, and which is capable of associating with a membrane; or
(d) an amino acid sequence comprising amino acids 27-110 of SEQ ID NO: 2 which is capable of associating with a membrane.