US 7,612,181 B2
	Dual variable domain immunoglobulin and uses thereof
Chengbin Wu, Shrewsbury, Mass. (US); Tariq Ghayur, Holliston, Mass. (US); Richard W. Dixon, Jefferson, Mass. (US); and Jochen G. Salfeld, North Grafton, Mass. (US)
Assigned to Abbott Laboratories, Abbott Park, Ill. (US)
Filed on Aug. 18, 2006, as Appl. No. 11/507,050.
Claims priority of provisional application 60/709911, filed on Aug. 19, 2005.
Claims priority of provisional application 60/732892, filed on Nov. 02, 2005.
Prior Publication US 2007/0071675 A1, Mar. 29, 2007
Int. Cl. C12P 21/08 (2006.01); C07K 10/00 (2006.01); A61K 39/00 (2006.01)

U.S. Cl. 530—387.3  [530/388.1; 530/391.3; 530/391.7; 424/178.1; 424/181.1; 424/183.1]

43 Claims

1. A binding protein comprising four polypeptide chains, wherein two polypeptide chains comprise VD1-(X1)n-VD2-C-(X2)n, wherein VD1 is a first heavy chain variable domain, VD2 is a second heavy chain variable domain, C is a heavy chain constant domain, X1 is a linker with the proviso that it is not CH1, and X2 is an Fc region; and two polypeptide chains comprise VD1-(X1)n-VD2-C-(X2)n, wherein VD1 is a first light chain variable domain, VD2 is a second light chain variable domain, C is a light chain constant domain, X1 is a linker with the proviso that it is not CH1, and X2 does not comprise an Fc region; and n is 0 or 1; wherein said four polypeptide chains of said binding protein form four functional antigen binding sites.