| US 7,612,019 B2 | ||
| Polypeptide display libraries and methods of making and using thereof | ||
| Patrick S. Daugherty, Santa Barbara, Calif. (US); Paul H. Bessette, Camarillo, Calif. (US); and Jeffrey Rice, Goleta, Calif. (US) | ||
| Assigned to The Regents of the University of California, Oakland, Calif. (US) | ||
| Filed on Dec. 19, 2006, as Appl. No. 11/612,757. | ||
| Application 11/612757 is a division of application No. 10/920244, filed on Aug. 18, 2004, granted, now 7,256,038. | ||
| Claims priority of provisional application 60/495698, filed on Aug. 18, 2003. | ||
| Prior Publication US 2007/0099247 A1, May 03, 2007 | ||
| Int. Cl. C40B 30/04 (2006.01); C40B 30/08 (2006.01); C40B 20/00 (2006.01); C40B 40/02 (2006.01); C12N 1/00 (2006.01) | ||
| U.S. Cl. 506—9 [506/11; 506/2; 506/14; 435/243] | 18 Claims |
| 1. An assay method for detecting a target in a sample, said method comprising:
contacting a passenger polypeptide with a sample suspected of containing said target, wherein said passenger polypeptide is
displayed on the surface of a biological entity, and wherein said passenger polypeptide is fused to an extracellular terminus
of a circularly permuted bacterial outer membrane protein; and detecting a specific interaction between the passenger polypeptide
and the target when present, wherein presence of the specific interaction is indicative of the presence of the target in the
sample.
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