US 7,611,881 B2
	Xylanse variants having altered sensitivity to xylanse inhibitors
Ole Sibbesen, Bagsvaerd (Denmark); and Jens Frisbaek Sørensen, Aarbus (Denmark)
Assigned to Danisco A/S, Copenhagen (Denmark)
Filed on Sep. 09, 2002, as Appl. No. 10/237,386.
Application 10/237386 is a continuation in part of application No. PCT/IB01/00426, filed on Mar. 08, 2001.
Claims priority of application No. 0005585.5 (GB), filed on Mar. 08, 2000; and application No. 0015751.1 (GB), filed on Jun. 27, 2000.
Prior Publication US 2003/0180895 A1, Sep. 25, 2003
Int. Cl. C12N 9/24 (2006.01); C12N 15/00 (2006.01); C12N 5/04 (2006.01); C12P 19/04 (2006.01); C12P 21/06 (2006.01); C07H 21/04 (2006.01)

U.S. Cl. 435—200  [435/101; 435/320.1; 435/419; 435/69.1; 536/23.2]

7 Claims

1. An isolated variant polypeptide or fragment thereof having at least 80% homology to SEQ ID NO: 1 as determined by using the GCG Wisconsin Bestfit package, and having xylanase activity, comprising two or more amino acid modifications, wherein said amino acid modifications are at two or more of positions 11, 12, 13, 15, 17, 29, 31, 32, 34, 113, 114, 119, 120, 121, 122, 123, 124 and 175 of the B. subtilis amino acid sequence shown as SEQ ID NO: 1, or their equivalent positions in other family 11 xylanases, such that the polypeptide or fragment thereof has altered sensitivity to a xylanase inhibitor as compared with the parent xylanase, wherein one of said modifications is at position 11, wherein the variant polypeptide is a family 11 xylanase, wherein said inhibitor is an inhibitor found naturally in wheat extract wherein said amino acid modifications are any of: D11Y, D11N, D11F, D11K, D11S, D11W, G12F, G13F, I15K, N17K, N17Y, N17D, N29K, N29Y, N29D, S31K, S31Y, S31D, N32K, G34D, G34F, G34T, Y113A, Y113D, Y113K, N114A, N114D, N114F, N114K, D119K, D119Y, D119N, G120K, G120D, G120F, G120Y, G120N, D121N, D121K, D121F, D121A, R122D, R122F, R122A, T123K, T123Y, T123D, T124K, T124Y, T124D, Q175E, Q175S and Q175L.