| US 7,611,847 B2 | ||
| Method for identifying an intestinal phenotype | ||
| Yoshiaki Ito, Singapore (Singapore); Kosei Ito, Singapore (Singapore); Hiroshi Fukamachi, Tokyo (Singapore); Hiroshi Ida, Singapore (Singapore); Chohei Sakakura, Kyoto (Japan); and Suk-Chul Bae, Cheongju (Korea, Republic of) | ||
| Assigned to Agency for Science, Technology and Research, Singapore (Singapore) | ||
| Filed on Jun. 01, 2006, as Appl. No. 11/445,925. | ||
| Claims priority of provisional application 60/686243, filed on Jun. 01, 2005. | ||
| Prior Publication US 2007/0015184 A1, Jan. 18, 2007 | ||
| Int. Cl. G01N 33/53 (2006.01) | ||
| U.S. Cl. 435—7.1 [435/7.21; 436/501; 436/518; 422/61] | 13 Claims |
| 1. A method for identifying a compound inhibiting the development of an intestinal phenotype in one or more gastric cells
which are in a precancerous state, the method comprising:
(a) contacting said one or more gastric cells with a solution supposed to contain at least one compound to be identified;
(b) incubating said cells for a predetermined period of time; and
(c) measuring the expression of the RUNX3 gene and the expression of said one or more intestinal marker genes,
wherein said intestinal phenotype is characterized by the loss of expression of the RUNX3 gene and a change in the expression
of one or more intestinal marker genes such that the level of said one or more marker genes is characteristic of said intestinal
phenotype;
wherein a compound inhibiting the development of an intestinal phenotype of one or more gastric cells is identified.
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