US 7,608,448 B2
	Method and cloning vectors utilizing intergeneric conjugation for manipulation of actinomycetes biosynthesis genes
Nathan Magarvey, Minneapolis, Minn. (US)
Assigned to Wyeth, Madison, N.J. (US)
Filed on Mar. 28, 2003, as Appl. No. 10/402,841.
Claims priority of provisional application 60/368712, filed on Mar. 29, 2002.
Prior Publication US 2003/0224484 A1, Dec. 04, 2003
Int. Cl. C12N 15/09 (2006.01); C12N 15/70 (2006.01); C12N 15/76 (2006.01); C12N 15/74 (2006.01)

U.S. Cl. 435—320.1  [435/69.1; 435/252.3; 435/252.33; 435/252.4]

18 Claims

1. An intergeneric shuttle vector comprising an expression control sequence; a multiple cloning site into which a nucleic acid encoding a polypeptide can be inserted that is positioned such that when the nucleic acid is inserted into the multiple cloning site the nucleic acid is operatively associated with the expression control sequence; an E. coli origin of replication; an actinomycetes origin of replication from pJV1; a cos cosmid cloning site; and an origin of transfer (OriT) that directs transfer of the vector from an E. coli cell to an actinomycetes cell during conjugation, and wherein the shuttle vector has a vector map according to pNWA200.