	US 7,608,420 B2
	Process for the recovery of staurosporine from a fermentation broth
Kurt Eyer, Visp (Switzerland); Georges Kalbermatten, Ausserberg (Switzerland); Jean-Peal Roduit, Sion (Switzerland); and Alain Wellig, Schweiz (Switzerland)
Assigned to Lonza AG, Basel (Switzerland)
Appl. No. 10/553,911 PCT Filed Apr. 19, 2004, PCT No. PCT/EP2004/004127 § 371(c)(1), (2), (4) Date Oct. 21, 2005, PCT Pub. No. WO2004/094645, PCT Pub. Date Nov. 04, 2004.
Claims priority of application No. 03009172 (EP), filed on Apr. 22, 2003.
Prior Publication US 2006/0194294 A1, Aug. 31, 2006
Int. Cl. C12P 1/00 (2006.01)

U.S. Cl. 435—41 [540/545]

16 Claims

1. A process for the recovery of staurosporine of formula:

from a fermentation broth comprising the steps of:

i) adding tetrahydrofuran to the fermentation broth;

ii) ultrafiltering the dilute fermentation broth obtained in step i);

iii) optionally diafiltrating the retenate obtained in step ii) with a mixture of a water and tetrahydrofuran, and optionally combining the permeates of the ultrafiltration and the diafiltration;

iv) optionally adjusting the pH of the permeate obtained in step ii) or step iii) to at least 8.5;

v) concentrating the permeate;

vi) adjusting the pH of the concentrate obtained in step v) to at least pH 8.5, unless the pH of said concentrate is already at least 8.5; and

vii) collecting the precipitated staurosporine obtained in step vi).