	US 7,608,405 B2
	Method of selectively assaying adiponectin multimers
Hiroyuki Ebinuma, Ryugasaki (Japan); Hirokazu Yago, Ryugasaki (Japan); Yuka Akimoto, Ryugasaki (Japan); Osamu Miyazaki, Ibaraki (Japan); Takashi Kadowaki, Tokyo (Japan); Toshimasa Yamauchi, Tokyo (Japan); and Kazuo Hara, Tokyo (Japan)
Assigned to Daiichi Pure Chemicals Co., Ltd., Tokyo (Japan); and Toudai TLO, Ltd., Tokyo (Japan)
Appl. No. 10/575,931 PCT Filed Oct. 15, 2004, PCT No. PCT/JP2004/015260 § 371(c)(1), (2), (4) Date Apr. 14, 2006, PCT Pub. No. WO2005/038457, PCT Pub. Date Apr. 28, 2005.
Claims priority of application No. 2003-354930 (JP), filed on Oct. 15, 2003.
Prior Publication US 2007/0042424 A1, Feb. 22, 2007
Int. Cl. G01N 33/53 (2006.01); G01N 33/543 (2006.01); A61K 39/395 (2006.01)

U.S. Cl. 435—7.1 [435/7.21; 436/501; 436/506; 436/518; 424/130.1; 424/178.1; 530/300; 530/350]

29 Claims

1. A method for selectively quantifying a target adiponectin multimer in a biological sample, comprising:

(a) selectively digesting at least one type of adiponectin multimer other than the target multimer with a protease, and

(b) immunologically quantifying the amount of the target adiponectin multimer.