| US 7,608,404 B2 | ||
| Disease determination method, data generation method for disease determination and data generation system for disease determination | ||
| Akimitsu Kakui, Hamamatsu (Japan); Katsu Honzawa, Hamamatsu (Japan); and Yukiko Sato, Hamamatsu (Japan) | ||
| Assigned to Hamamatsu Photonics K.K., Hamamatsu-shi, Shizuoka (Japan) | ||
| Appl. No. 10/533,116 PCT Filed Oct. 30, 2003, PCT No. PCT/JP03/13935 § 371(c)(1), (2), (4) Date Jan. 23, 2006, PCT Pub. No. WO2004/040273, PCT Pub. Date May 13, 2004. |
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| Claims priority of application No. 2002-320044 (JP), filed on Nov. 01, 2002. | ||
| Prior Publication US 2006/0234319 A1, Oct. 19, 2006 | ||
| Int. Cl. G01N 33/53 (2006.01); G01N 33/566 (2006.01) | ||
| U.S. Cl. 435—7.1 [435/7.92; 436/501; 436/506; 436/518; 424/130.1; 424/178.1; 530/300; 530/350] | 13 Claims |
| 1. Data generation method for disease determination for generating data for disease determination using a biological sample
obtained from the subject, comprising;
pretreatment process to add an acidic or alkaline solution to the sample and to heat the sample,
excitation light irradiation process to irradiate the sample with the excitation light and to continuously or intermittently
change the wavelength of the excitation light,
emission light measurement process to measure the wavelength and intensity of the emission light emitted from the sample in
response to the excitation light, and
analyzing and sorting process to detect a specific point in the three-dimensional optical spectrum composed of the excitation
light wavelength, emission light wavelength and emission light intensity and to sort the sample based on an attribute of the
specific point, wherein the specific point is a relative maximum peak or a relative minimum peak of a fluorescence intensity
and is utilized for disease determination.
|