| US 7,608,248 B2 | ||
| Stable isotope-labeled aromatic amino acids, method for incorporating the same in target protein and method for NMR-structural analysis of proteins | ||
| Masatsune Kainosho, Tokyo (Japan); and Tsutomu Terauchi, Sagamihara (Japan) | ||
| Assigned to Japan Science and Technology Agency, Saitama (Japan) | ||
| Filed on Apr. 28, 2006, as Appl. No. 11/414,756. | ||
| Application 11/414756 is a continuation of application No. PCT/JP2004/016215, filed on Nov. 01, 2004. | ||
| Claims priority of application No. 2003-373304 (JP), filed on Oct. 31, 2003. | ||
| Prior Publication US 2006/0194328 A1, Aug. 31, 2006 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. A61B 5/055 (2006.01); C12P 13/04 (2006.01); C12P 9/00 (2006.01); C07K 1/00 (2006.01) | ||
| U.S. Cl. 424—9.34 [435/106; 435/131; 530/350] | 2 Claims |
| 1. A method for the NMR spectroscopic structural analysis of a target protein comprising the steps of incorporating into the
target protein stable isotope-labeled amino acids selected from the group consisting of:
A stable isotope-labeled phenylalanine represented by the following formulae (1) to (3); and
A stable isotope-labeled tyrosine represented by the following formulae (5) to (8); and then carrying out NMR spectroscopy on the target protein to perform the structural analysis on the target protein
  |