| US 7,604,807 B2 | ||
| Use of pullulan to isolate and preserve biological material | ||
| Vitaly J. Vodyanoy, Auburn, Ala. (US); James M. Barbaree, Dadeville, Ala. (US); Bryan A. Chin, Auburn, Ala. (US); William Charles Neely, Auburn, Ala. (US); Suram T. Pathirana, Sunnyvale, Calif. (US); and Timothy D. Braden, Auburn, Ala. (US) | ||
| Assigned to Auburn University, Auburn, Ala. (US) | ||
| Filed on Jan. 27, 2006, as Appl. No. 11/341,152. | ||
| Application 11/341152 is a continuation in part of application No. 10/000727, filed on Nov. 30, 2001, granted, now 7,022,514. | ||
| Claims priority of provisional application 60/250798, filed on Dec. 01, 2000. | ||
| Claims priority of provisional application 60/250799, filed on Dec. 01, 2000. | ||
| Prior Publication US 2006/0172332 A1, Aug. 03, 2006 | ||
| Int. Cl. C12N 1/04 (2006.01); C12N 5/06 (2006.01); C12Q 1/68 (2006.01); A01N 63/02 (2006.01) | ||
| U.S. Cl. 424—195.18 [435/4; 435/260] | 14 Claims |
| 1. A solid containing a reversibly preserved microorganism in a dormant and preserved state comprising:
a cured suspension comprising the microorganism in an isolated condition combined with an effective amount of a pullulan solution,
wherein said suspension is cured under ambient conditions to form a solid containing said microorganism in a dormant and preserved
state;
wherein the suspension can be restored by irrigating said solid under ambient conditions with an effective amount of an aqueous
solution to restore said suspension and the suspension can be separated such that said microorganism is restored to said isolated
condition.
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