US 7,601,891 B2
	Optimizing glycan processing plants
Hendrikus Antonius Cornelis Bakker, Hannover (Germany); Hendrik Jan Bosch, Wageningen (Netherlands); Dionisius Elisabeth Antonius Florack, Wageningen (Netherlands); and Gerard Johan Adolph Rouwendal, Heteren (Netherlands)
Assigned to Plant Research International B.V., Wageningen (Netherlands)
Appl. No. 10/508,165 PCT Filed Mar. 18, 2003, PCT No. PCT/IB03/01526 § 371(c)(1), (2), (4) Date Sep. 17, 2004, PCT Pub. No. WO03/078637, PCT Pub. Date Sep. 25, 2003.
Claims priority of provisional application 60/365735, filed on Mar. 19, 2002.
Prior Publication US 2006/0253928 A1, Nov. 09, 2006
Int. Cl. C12N 15/62 (2006.01); C12N 15/63 (2006.01); C12N 15/82 (2006.01)

U.S. Cl. 800—284  [800/298; 800/288; 435/320.1; 435/468; 536/23.4]

2 Claims

1. A method of producing a plant cell expressing a heterologous glycoprotein that is reduced in xylose and fucose, comprising introducing into the plant cell a first expression cassette and a second expression cassette, wherein the first expression cassette comprises a nucleic acid comprising SEQ ID No:3 encoding a hybrid enzyme, and the second expression cassette comprises nucleic acid encoding a heterologous glycoprotein, and expressing the hybrid enzyme and the heterologous glycoprotein, wherein the expressed glycoprotein is reduced in xylose and fucose when compared to a glycoprotein expressed in a plant cell in the absence of the hybrid enzyme.