| US 7,601,685 B2 | ||
| Growth factor modified protein matrices for tissue engineering | ||
| Jeffrey A. Hubbell, Zurich (Switzerland); Jason C. Schense, Zurich (Switzerland); and Shelly E. Sakiyama-Elbert, St. Louis, Mo. (US) | ||
| Assigned to Eidgenossische Technische Hochschule Zurich, Zurich (Switzerland); and Universitat Zurich, Zurich (Switzerland) | ||
| Filed on Dec. 17, 2002, as Appl. No. 10/323,046. | ||
| Application 10/323046 is a continuation in part of application No. 09/563760, filed on May 01, 2000. | ||
| Application 09/563760 is a continuation in part of application No. 09/141153, filed on Aug. 27, 1998, abandoned. | ||
| Prior Publication US 2003/0187232 A1, Oct. 02, 2003 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. A61K 38/00 (2006.01) | ||
| U.S. Cl. 514—2 [514/12; 514/17; 530/350; 530/300] | 13 Claims |
| 1. A fusion protein, comprising:
(i) a first protein domain;
(ii) a second protein domain; and
(iii) an enzymatic or hydrolytic cleavage site between the first and the second domains;
wherein the first domain is a growth factor selected from the group consisting of the platelet derived growth factor superfamily
and the transforming growth factor beta (TGFβ) superfamily;
wherein the second domain is a crosslinking Factor XIIIa substrate domain;
wherein the enzymatic cleavage site is selected from the group consisting of proteolytic substrates and polysaccharide substrates,
and
wherein the hydrolytic cleavage site comprises a substrate with a linkage which undergoes hydrolysis by an acid or a base
catalyzed reaction.
|