US 7,601,523 B2
Method for production of arachidonic acid
William R. Barclay, Boulder, Colo. (US)
Assigned to Martek Biosciences Corporation, Columbia, Md. (US)
Filed on Jul. 24, 2007, as Appl. No. 11/782,566.
Application 09/972550 is a division of application No. 09/480060, filed on Jan. 10, 2000, granted, now 6,319,698, filed on Nov. 20, 2001.
Application 08/763973 is a division of application No. 08/377766, filed on Jan. 24, 1995, granted, now 5,583,019, filed on Dec. 10, 1996.
Application 11/782566 is a continuation of application No. 11/531439, filed on Sep. 13, 2006.
Application 11/531439 is a continuation of application No. 10/802392, filed on Mar. 04, 2004, granted, now 7,195,791, filed on Mar. 27, 2007.
Application 10/802392 is a continuation of application No. 09/972550, filed on Oct. 05, 2001, granted, now 6,749,849, filed on Jul. 24, 2007.
Application 09/480060 is a continuation of application No. 09/270294, filed on Mar. 15, 1999, granted, now 6,245,365, filed on Jun. 12, 2001.
Application 09/270294 is a continuation of application No. 08/763973, filed on Dec. 10, 1996, granted, now 5,882,703, filed on Mar. 16, 1999.
Prior Publication US 2008/0044388 A1, Feb. 21, 2008
Int. Cl. C12P 7/64 (2006.01); C12N 1/00 (2006.01)
U.S. Cl. 435—134  [435/254.1] 13 Claims
 
1. A method of recovering arachidonic acid-containing lipids, comprising
culturing microorganisms of the genus Mortierella sect. schmuckeri in a fermentation medium,
harvesting the microorganisms,
recovering lipids from the microorganisms, and
removing impurities from the recovered lipids, whereby arachidonic acid-containing lipids are recovered; wherein said Mortierella sect. schmuckeri produces at least about 0.70 grams of arachidonic acid per liter per day when grown under appropriate fermentation conditions.