US 7,601,513 B2
Recombinant methods for expressing a functional umami (T1R1/T1R3) taste receptor
Mark Zoller, San Diego, Calif. (US); Xiaodong Li, San Diego, Calif. (US); Lena Staszewski, San Diego, Calif. (US); Shawn O'Connell, Encinitas, Calif. (US); Sergey Zozulya, San Diego, Calif. (US); Jon Elliot Adler, San Diego, Calif. (US); Hong Xu, San Diego, Calif. (US); and Femando Echeverri, Chula Vista, Calif. (US)
Assigned to Senomyx, Inc., San Diego, Calif. (US)
Filed on Dec. 03, 2003, as Appl. No. 10/725,473.
Application 10/725473 is a division of application No. 10/179373, filed on Jun. 26, 2002, granted, now 7,368,285.
Application 10/179373 is a continuation in part of application No. 10/035045, filed on Jan. 03, 2002, granted, now 7,241,880.
Application 10/035045 is a continuation in part of application No. 09/897427, filed on Jul. 03, 2001, granted, now 6,955,887.
Application 09/897427 is a continuation in part of application No. 09/799629, filed on Mar. 07, 2001, granted, now 7,244,835.
Claims priority of provisional application 60/374143, filed on Apr. 22, 2002.
Claims priority of provisional application 60/372090, filed on Apr. 15, 2002.
Claims priority of provisional application 60/339472, filed on Dec. 14, 2001.
Claims priority of provisional application 60/331771, filed on Nov. 21, 2001.
Claims priority of provisional application 60/310493, filed on Aug. 08, 2001.
Claims priority of provisional application 60/304749, filed on Jul. 13, 2001.
Claims priority of provisional application 60/300434, filed on Jun. 26, 2001.
Prior Publication US 2008/0050778 A1, Feb. 28, 2008
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 21/06 (2006.01); C12N 1/20 (2006.01); C12N 15/74 (2006.01)
U.S. Cl. 435—69.1  [435/252.3; 435/471] 39 Claims
 
1. A method of producing a heteromeric taste receptor comprising:
expressing at least one T1R1 nucleic acid sequence and at least one T1R3 nucleic acid sequence in a recombinant host cell under conditions which result in a heteromeric taste receptor comprising at least one T1R1 and T1R3 polypeptide, wherein said T1R1 polypeptide is (i) encoded by a nucleic acid sequence comprising SEQ. ID. NO: 8, (ii) encoded by a nucleic acid sequence comprising a nucleic acid that hybridizes to SEQ. ID. NO: 8 under stringent hybridization conditions which are conducting the hybridization reaction at 42° C. in a solution comprising 50% formamide, 5X SSC, and 1% SDS and washing at 65° C. in a solution comprising 0.2X SSC and 0.1% SDS, or (iii) a T1R1 polypeptide possessing at least 95% sequence identity to SEQ. ID. NO: 5;
and wherein said T1R3 polypeptide is (i) encoded by a nucleic acid sequence comprising SEQ. ID. NO: 9; (ii) encoded by a nucleic acid sequence that hybridizes to SEQ. ID. NO: 9 under stringent hybridization conditions which are conducting the hybridization reaction at 42° C. in a solution comprising 50% formamide, 5X SSC, 10% SDS; and washing at 65° C. in a solution comprising 0.2X SCC and 0.1% SDS, or (iii) a T1R3 polypeptide possessing at least 95% sequence identity to SEQ. ID. NO: 7.