| US 7,601,507 B2 | ||
| Electronic methods for the detection of analytes | ||
| Stephen D. O'Connor, Pasadena, Calif. (US); Jon Faiz Kayyem, Pasadena, Calif. (US); and Thomas J. Meade, Wilmette, Ill. (US) | ||
| Assigned to Osmetech Technology Inc., Pasadena, Calif. (US) | ||
| Filed on Oct. 30, 2007, as Appl. No. 11/981,645. | ||
| Application 11/981645 is a division of application No. 09/096593, filed on Jun. 12, 1998, granted, now 7,560,237. | ||
| Claims priority of provisional application 60/049489, filed on Jun. 12, 1997. | ||
| Prior Publication US 2009/0173640 A1, Jul. 09, 2009 | ||
| Int. Cl. G01N 33/53 (2006.01) | ||
| U.S. Cl. 435—7.1 [435/7.21; 436/501; 436/518] | 22 Claims |
| 1. A method of detecting a protein target analyte in a sample comprising:
a) applying a first input signal to an assay complex comprising:
i) a redox active complex comprising:
1) a redox active molecule;
2) a binding ligand that will bind a protein target analyte; and
ii) said protein target analyte,
wherein said binding ligand is covalently attached to a first measuring electrode via a spacer, and wherein said first measuring
electrode comprises:
a passivation agent monolayer comprising at least a covalently attached first passivation species and a covalently attached
second passivation species comprising said binding ligand; and
b) detecting a change in the faradaic impedance of the system as a result of the association of the redox active molecule
with said protein target analyte, if present.
|