| US 7,599,059 B2 | ||
| Monitoring molecular interactions using photon arrival-time interval distribution analysis | ||
| Ted A. Laurence, Livermore, Calif. (US); and Shimon Weiss, Los Angels, Calif. (US) | ||
| Assigned to The Regents of the University of California, Oakland, Calif. (US) | ||
| Appl. No. 10/521,632 PCT Filed Jul. 25, 2003, PCT No. PCT/US03/23252 § 371(c)(1), (2), (4) Date Feb. 09, 2006, PCT Pub. No. WO2004/011903, PCT Pub. Date Feb. 05, 2004. |
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| Claims priority of provisional application 60/398955, filed on Jul. 25, 2002. | ||
| Prior Publication US 2006/0176479 A1, Aug. 10, 2006 | ||
| Int. Cl. G01J 3/30 (2006.01) | ||
| U.S. Cl. 356—317 | 18 Claims |
| 1. A method for analyzing properties of one or more species that are labeled with fluorophores, said method comprising the
steps of:
using a detector to detect a plurality of photons that are emitted in a photon stream from a species that is labeled with
a fluorophore located in a detection volume wherein each of said photons arrives at said detector at an arrival time;
determining the arrival time of each of said photons in said plurality of photons;
identifying the intervals between the arrival time of a given photon and the arrival time of other photons in said plurality
of photons to thereby provide photon pair intervals that are a measure of the time between the arrival of each pair of photons
in said plurality of photons;
determining the number of photons that have arrival times that are within said photon pair intervals to provide a measure
of intervening photons located within said photon pair intervals; and
calculating properties of said species that are located in said detection volume based on a relationship between said photon
pair intervals and said measure of intervening photons.
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