| US 7,598,073 B2 | ||
| Methods for producing high yields of zygotic-like cotyledonary pine embryos utilizing media that include a disaccharide and glucose | ||
| Pramod K. Gupta, Federal Way, Wash. (US); Diane Gail Holmstrom, Bonney Lake, Wash. (US); and Bonnie Larson, Granite Falls, Wash. (US) | ||
| Assigned to Weyerhaeuser NR Company, Federal Way, Wash. (US) | ||
| Filed on Mar. 21, 2003, as Appl. No. 10/394,549. | ||
| Prior Publication US 2004/0003426 A1, Jan. 01, 2004 | ||
| Int. Cl. A01H 4/00 (2006.01); A01H 7/00 (2006.01) | ||
| U.S. Cl. 435—240.45 [435/240.4; 435/240.46; 435/240.47; 435/240.49; 435/240.54; 435/172.1] | 26 Claims |
| 1. A method for producing cotyledonary pine embryos, said method comprising the steps of:
(a) culturing embryogenic pine tissue in, or on, a maintenance medium comprising maltose as the sole source of sugar to promote
multiplication of the embryogenic tissue; and
(b) culturing the embryogenic pine tissue multiplied in accordance with step (a) in, or on, a development medium comprising
maltose and glucose to yield cotyledonary pine embryos, wherein said maltose is present in the development medium at a concentration
of 2.5% and said glucose is present in the development medium at a concentration of 1%.
|