| US 7,595,194 B2 | ||
| Isolation of mammalian CNS glial-restricted precursor cells | ||
| Mahendra S. Rao, Salt Lake City, Utah (US); and Margot Mayer-Proschel, Sandy, Utah (US) | ||
| Assigned to University of Utah Research Foundation, Salt Lake City, Utah (US) | ||
| Filed on May 01, 2006, as Appl. No. 11/415,056. | ||
| Application 11/415056 is a division of application No. 10/025333, filed on Dec. 19, 2001, granted, now 7,037,720. | ||
| Application 10/025333 is a continuation of application No. 08/852744, filed on May 07, 1997, granted, now 6,361,996. | ||
| Prior Publication US 2006/0199264 A1, Sep. 07, 2006 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. C12N 5/06 (2006.01) | ||
| U.S. Cl. 435—368 [435/378; 435/402; 435/375] | 3 Claims |
| 1. A method of isolating a pure population of mammalian CNS glial-restricted precursor cells wherein said cells are capable
of self-renewal in adherent feeder-cell-independent culture medium and of differentiation to CNS glial cells but not CNS neuronal
cells, comprising the steps of:
(a) isolating a population of mammalian CNS neuroepithelial stems cells;
(b) incubating the neuroepithelial stem cells in a medium lacking an effective amount of chick embryo extract for a period
of time sufficient for the cells to begin differentiating;
(c) subjecting the incubated cells to specific antibody capture using an A2B5 antibody characteristic of glial-restricted
precursor cells to result in a captured subpopulation of cells; and
(d) incubating the captured subpopulation of cells in a medium configured for supporting adherent growth thereof comprising
effective amounts of fibroblast growth factor and platelet derived growth factor.
|