| US 7,595,181 B2 | ||
| Thermostable esterases from thermophilic bacteria | ||
| Burghard Grüning, Essen (Germany); Geoffrey Hills, Essen (Germany); Thomas Veit, Hagen (Germany); Christian Weitemeyer, Essen (Germany); Olivier Favre-Bulle, Nimes (France); Fabrice Lefevre, Nimes (France); Hong-Khanh Nguyen, Nimes (France); and Gilles Ravot, Nimes (France) | ||
| Assigned to Proteus S.A., (France) | ||
| Filed on Sep. 30, 2004, as Appl. No. 10/954,826. | ||
| Claims priority of application No. 03021972 (EP), filed on Sep. 30, 2003. | ||
| Prior Publication US 2005/0112644 A1, May 26, 2005 | ||
| Int. Cl. C12N 9/18 (2006.01); C07K 14/195 (2006.01); C12P 21/02 (2006.01); C12P 7/64 (2006.01) | ||
| U.S. Cl. 435—197 [530/350; 435/69.1; 435/134] | 10 Claims |
| 1. An isolated thermostable esterase comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:3
or at least 95% sequence identity to SEQ ID NO:4,
wherein said esterase has a catalyzing activity in a temperature range of 70° C. to 95° C., and
wherein said esterase retains at least 80% specific esterase activity as measured by the hydrolysis of a substrate selected
from the group consisting of 2-hydroxy-4-p-nitrophenoxy-butyl decanoate and 2-hydroxy-4-p-nitrophenoxy-butyl propionate.
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