US 8,537,356 B2
Opto-fluidic nanoparticle detection apparatus
H. Daniel Ou-Yang, Bethlehem, Pa. (US); and Xuanhong Cheng, Bethlehem, Pa. (US)
Assigned to Lehigh University, Bethlehem, Pa. (US)
Appl. No. 12/920,262
Filed by H. Daniel Ou-Yang, Bethlehem, Pa. (US); and Xuanhong Cheng, Bethlehem, Pa. (US)
PCT Filed Mar. 2, 2009, PCT No. PCT/US2009/035683
§ 371(c)(1), (2), (4) Date Jan. 3, 2011,
PCT Pub. No. WO2009/108921, PCT Pub. Date Sep. 3, 2009.
Claims priority of provisional application 61/032,184, filed on Feb. 28, 2008.
Prior Publication US 2011/0085166 A1, Apr. 14, 2011
Int. Cl. G01N 21/00 (2006.01)
U.S. Cl. 356—338  [356/300; 356/344; 356/369; 250/225] 19 Claims
OG exemplary drawing
 
1. A method for manipulating a plurality of nanoparticles comprising: providing a first suspension of fluorescent labeled nanoparticles in a liquid solution; placing a portion of the first suspension within a reservoir; providing a first laser beam from a first laser source and a second laser beam from a second laser source; applying the first laser beam through a focusing optical element and into the reservoir to thereby establish an optical gradient and forming an optical trap within the reservoir; applying the second laser beam to the optical trap to thereby produce fluorescence optical signal from the fluorescent labeled nanoparticles within the optical trap; and detecting the fluorescence optical signal with a photodetector; and processing the detected fluorescence optical signal to determine at least one parameter selected from the group consisting of: the quantity of nanoparticles in the first suspension; the quantity of the nanoparticles in the optical trap; the concentration of nanoparticles in the first suspension; the concentration of the nanoparticles in the optical trap; the osmotic compressibility of the nanoparticles in the first suspension; and the osmotic compressibility of the nanoparticles in the optical trap.