US 7,592,322 B2
	RNAi modulation of RSV, PIV and other respiratory viruses and uses thereof
Sailen Barik, Mobile, Ala. (US)
Assigned to Alnylam Pharmaceuticals, Inc., Cambridge, Mass. (US)
Filed on Jun. 14, 2005, as Appl. No. 11/151,893.
Claims priority of provisional application 60/621552, filed on Oct. 22, 2004.
Prior Publication US 2006/0089323 A1, Apr. 27, 2006
Int. Cl. A61K 31/70 (2006.01); C07H 21/04 (2006.01)

U.S. Cl. 514—44  [536/24.5]

6 Claims

1. A method of reducing the levels of a viral titer from a Respiratory Syncytial Virus (RSV) and a Parainfluenza Virus (PIV) in a cell of the respiratory tract of a subject comprising the step of co-administering intranasally a first and a second siRNA agent to said subject, wherein said first siRNA agent comprises a sense strand consisting of SEQ ID NO: 4 and an antisense strand consisting of SEQ ID NO:3; and wherein said second siRNA agent comprises a sense strand consisting of 21-23 contiguous nucleotides wherein at least 19 nucleotides are identical to SEQ ID NO: 10, and an antisense strand consisting of 21-23 contiguous nucleotides, wherein at least 19 nucleotides are identical to SEQ ID NO: 9, and wherein 19-21 contiguous nucleotides of said antisense strand of said second siRNA agent are complementary to a target RNA from PIV.