US 7,592,171 B2
	Vibrio cholerae with improved biological safety features in freeze dried form
Javier Campos Gómez, Santa Clara (Cuba); Tomas Marcelino Moreira Hernandez, Habana (Cuba); Boris Luis Rodriguez Gonzalez, Habana (Cuba); Karen Marrero Dominguez, Habana (Cuba); Eriel Martinez Gutierrez, Habana (Cuba); Talena Yamile Ledon Perez, Habana (Cuba); Yussuan Silva Larrañaga, Habana (Cuba); Edith Suzarte Portal, Habana (Cuba); Herminia de la Caridad Delgado Rodriguez, Habana (Cuba); Caridad Urra Villavicencio, Habana (Cuba); and Rafael Alfredo Fando Calzada, Habana (Cuba)
Assigned to Centro Nacional de Investigaciones Cientificas (CNIC), Havana (Cuba)
Appl. No. 10/546,410 PCT Filed Feb. 19, 2004, PCT No. PCT/CU2004/000002 § 371(c)(1), (2), (4) Date Jul. 03, 2006, PCT Pub. No. WO2004/073736, PCT Pub. Date Sep. 02, 2004.
Claims priority of application No. 2003-0039 (CU), filed on Feb. 20, 2003; and application No. 2003-0084 (CU), filed on Apr. 17, 2003.
Prior Publication US 2007/0071775 A1, Mar. 29, 2007
Int. Cl. C12N 1/04 (2006.01); C12N 1/20 (2006.01); A61K 39/106 (2006.01)

U.S. Cl. 435—252.1  [435/260; 424/261.1]

3 Claims

1. A freeze-dried composition comprising one or more living attenuated strains of Vibrio cholerae, wherein the one or more strains of Vibrio cholerae comprise at least one mutation inactivating at least one gene essential for the biogenesis of mannose-sensitive hemagglutinin fimbria (MSHA), and further lack sequence of the VGJΦ phage (SEQ ID NO: 1), and the composition contains lactose sorbitol, and either peptone or yeast extract at a total concentration not higher than 10%.