US 7,592,168 B2
	Cephalosporin C acylase mutant and method for preparing 7-ACA using same
Yong Chul Shin, Jinju-si (Korea, Republic of); John Y J Jeon, Seoul (Korea, Republic of); Kyung Hwa Jung, Jinju-si (Korea, Republic of); Mi Ran Park, Busan (Korea, Republic of); and Youngso Kim, Seoul (Korea, Republic of)
Assigned to Sandoz AG, Basel (Switzerland)
Appl. No. 10/568,132 PCT Filed Aug. 10, 2004, PCT No. PCT/KR2004/002005 § 371(c)(1), (2), (4) Date Oct. 19, 2006, PCT Pub. No. WO2005/014821, PCT Pub. Date Feb. 17, 2005.
Claims priority of application No. 10-2003-0055259 (KR), filed on Aug. 11, 2003.
Prior Publication US 2007/0207519 A1, Sep. 06, 2007
Int. Cl. C12N 9/80 (2006.01); C07H 21/04 (2006.01)

U.S. Cl. 435—228  [536/23.2]

17 Claims

1. A CPC acylase mutant, wherein at least one amino acid selected from the group consisting of Val121α, Gly 139α and Phe 169α of CPC acylase α-subunit of SEQ ID NO: 4 and Phe 58β, His 70β, Ile 75β, Ile 176β and Ser 471β of CPC acylase β-subunit of SEQ ID NO: 5 is replaced by another amino acid.