	US 11,753,672 B2
	Swab sample nucleic acid releaser and use thereof
Yunshao He, Guangdong (CN); Xiwen Jiang, Guangdong (CN); Qiao Xia, Guangdong (CN); Zhiqiang Dong, Guangdong (CN); Lili Liao, Guangdong (CN); and Hanrong Li, Guangdong (CN)
Assigned to DA AN GENE CO., LTD., Guangdong (CN)
Appl. No. 17/284,476
Filed by DA AN GENE CO., LTD., Guangdong (CN)
PCT Filed Oct. 27, 2020, PCT No. PCT/CN2020/124134 § 371(c)(1), (2) Date Apr. 12, 2021, PCT Pub. No. WO2021/248779, PCT Pub. Date Dec. 16, 2021.
Claims priority of application No. 202010538226.2 (CN), filed on Jun. 12, 2020.
Prior Publication US 2022/0145356 A1, May 12, 2022
Int. Cl. C12Q 1/6806 (2018.01)

CPC C12Q 1/6806 (2013.01)

3 Claims

1. A swab sample nucleic acid releaser, comprising the following components:

(1) 1 mol/L of a metal ion chelating agent;

(2) 40 mmol/L of a buffer at pH 9;

(3) 40% (v/v) of a polar organic solvent;

(4) 1% (v/v) of a surfactant; and

(5) 1 mol/L of a nuclease inhibitor, wherein the metal ion chelating agent is citric acid; the buffer is a sodium phosphate buffer; the polar organic solvent is hexamethylphosphoramide; the surfactant is SDS; and the nuclease inhibitor is 4,4′-carbonylbis(2-(1-naphthoylamino)benzoic acid).