US 11,753,659 B2
Glycerol and acetic acid converting yeast cells with improved acetic acid conversion
Paul Klaassen, Echt (NL); and Wouter Willem Antonius Hartman, Echt (NL)
Assigned to DSM IP ASSETS B.V., Heerlen (NL)
Filed by DSM IP ASSETS B.V., Heerlen (NL)
Filed on Jul. 12, 2019, as Appl. No. 16/509,700.
Application 16/509,700 is a continuation of application No. 14/914,612, granted, now 10,450,588, previously published as PCT/EP2014/068324, filed on Aug. 29, 2014.
Claims priority of application No. 13182222 (EP), filed on Aug. 29, 2013.
Prior Publication US 2019/0330664 A1, Oct. 31, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 7/10 (2006.01); C12N 1/16 (2006.01); C12N 9/04 (2006.01); C12N 9/02 (2006.01); C12N 9/00 (2006.01); C12P 7/06 (2006.01); C12N 9/54 (2006.01); C12N 9/12 (2006.01)
CPC C12P 7/10 (2013.01) [C12N 1/16 (2013.01); C12N 9/0006 (2013.01); C12N 9/0008 (2013.01); C12N 9/1205 (2013.01); C12N 9/54 (2013.01); C12N 9/93 (2013.01); C12P 7/06 (2013.01); C12Y 101/01006 (2013.01); C12Y 102/0101 (2013.01); C12Y 207/01028 (2013.01); C12Y 207/01029 (2013.01); C12Y 602/01001 (2013.01); Y02E 50/10 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A yeast cell that is genetically modified comprising:
a) one or more nucleotide sequence encoding a heterologous ethanolamine utilization protein having the amino acid sequence set forth in SEQ ID NO: 3 or a functional homologue thereof having at least 80% amino acid identity to SEQ ID NO: 3;
b) one or more nucleotide sequence encoding a acetyl-CoA synthetase (belonging to E.C. 6.2.1.1);
c) one or more nucleotide sequence encoding a glycerol dehydrogenase (belonging to E.C. 1.1.1.6); and
d) one or more nucleotide sequence encoding a dihydroxyacetone kinase (belonging to E.C. 2.7.1.28 or E.C. 2.7.1.29), wherein the one or more recombinant nucleotide sequence encoding the dihydroxyacetone kinase is overexpressed in the yeast cell, and
wherein the yeast cell consumes glucose, xylose, glycerol and acetic acid.