US 11,753,634 B2
Method and apparatus for applying heat to living tissue
Chih-Yu Chao, Taipei (TW); Wei-Ting Chen, Taipei (TW); Chueh-Hsuan Lu, Taipei (TW); Chih-Hsiung Hsieh, Taipei (TW); Yu-Yi Kuo, Taipei (TW); Guan-Bo Lin, Taipei (TW); and Yi-Kun Sun, Taipei (TW)
Assigned to NATIONAL TAIWAN UNIVERSITY, Taipei (TW)
Filed by NATIONAL TAIWAN UNIVERSITY, Taipei (TW)
Filed on Jan. 10, 2020, as Appl. No. 16/739,746.
Claims priority of application No. 108145555 (TW), filed on Dec. 12, 2019.
Prior Publication US 2021/0180040 A1, Jun. 17, 2021
Int. Cl. A01N 1/02 (2006.01); C12N 13/00 (2006.01); C12N 5/071 (2010.01); C12N 5/0793 (2010.01)
CPC C12N 13/00 (2013.01) [A01N 1/0284 (2013.01); C12N 5/0619 (2013.01); C12N 5/0697 (2013.01)] 8 Claims
OG exemplary drawing
 
1. A method for applying heat to living tissue, comprising the following steps:
S1) high temperature: in a first time interval, heat at least a portion of a living tissue, and maintain a temperature of the at least a portion of the living tissue between 39-46° C. within the first time interval; the first time interval being less than 30 minutes; wherein the at least a portion of the living tissue includes a plurality of normal cells and a plurality of abnormal cells;
S2) low temperature: use an end-time point of the first time interval as a starting point of a second time interval, and cool the at least a portion of the living tissue in the second time interval; and a temperature of the least a portion of the living tissue in the second time interval being lower than a maximum temperature in the first time interval; and a time period required for the at least a portion of the living tissue to be cooled from 45° C. to 39° C. via a natural cooling method under a criteria where an environmental temperature is between 36.5-37° C. being defined to be a natural cooling time interval; the second time interval being shorter than the natural cooling time interval; and
S3) high temperature: use an end-time point of the second time interval as a starting point of a third time interval, and heat the at least a portion of the living tissue in the third time interval in order to increase a temperature of the at least a portion of the living tissue, and maintain the temperature of the at least a portion of the living tissue to be between 39-46° C. in the third time interval; the third time interval being less than 30 minutes; the second time interval being shorter than the first time interval, and the second time interval being shorter than the third time interval.