CPC B01J 19/0046 (2013.01) [C12Q 1/6874 (2013.01); B01J 2219/0059 (2013.01); B01J 2219/00709 (2013.01); B01J 2219/00722 (2013.01); C40B 40/06 (2013.01); C40B 50/06 (2013.01)] | 13 Claims |
1. A nucleic acid comprising:
(a) a first nucleic acid strand comprising: (i) a first sequence comprising at least 6 random bases; (ii) a second sequence comprising a nucleic acid sample sequence, wherein the second sequence comprises at least 200 bases; and (iii) a 3′ terminal end that cannot support strand extension; and
(b) a second nucleic acid strand, comprising at least 6 random bases, wherein a 3′ end of the second nucleic acid strand is annealed to the first nucleic acid strand at an internal position on the first nucleic acid strand.
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