US 11,752,483 B2
Methods of sample preparation
Steven Robert Head, La Jolla, CA (US); Phillip T. Ordoukhanian, La Jolla, CA (US); and Daniel R. Salomon, La Jolla, CA (US)
Assigned to The Scripps Research Institute, La Jolla, CA (US)
Filed by The Scripps Research Institute, La Jolla, CA (US)
Filed on Aug. 10, 2020, as Appl. No. 16/989,791.
Application 16/989,791 is a continuation of application No. 15/948,459, filed on Apr. 9, 2018, granted, now 10,780,412.
Application 15/948,459 is a continuation of application No. 14/549,411, granted, now 9,968,901, issued on May 15, 2018, previously published as PCT/US2013/042106, filed on May 21, 2013.
Claims priority of provisional application 61/649,836, filed on May 21, 2012.
Claims priority of provisional application 61/654,389, filed on Jun. 1, 2012.
Claims priority of provisional application 61/716,378, filed on Oct. 19, 2012.
Claims priority of provisional application 61/749,871, filed on Jan. 7, 2013.
Claims priority of provisional application 61/763,424, filed on Feb. 11, 2013.
Claims priority of provisional application 61/763,441, filed on Feb. 11, 2013.
Prior Publication US 2021/0121845 A1, Apr. 29, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. B01J 19/00 (2006.01); C12Q 1/6874 (2018.01); C40B 40/06 (2006.01); C40B 50/06 (2006.01)
CPC B01J 19/0046 (2013.01) [C12Q 1/6874 (2013.01); B01J 2219/0059 (2013.01); B01J 2219/00709 (2013.01); B01J 2219/00722 (2013.01); C40B 40/06 (2013.01); C40B 50/06 (2013.01)] 13 Claims
 
1. A nucleic acid comprising:
(a) a first nucleic acid strand comprising: (i) a first sequence comprising at least 6 random bases; (ii) a second sequence comprising a nucleic acid sample sequence, wherein the second sequence comprises at least 200 bases; and (iii) a 3′ terminal end that cannot support strand extension; and
(b) a second nucleic acid strand, comprising at least 6 random bases, wherein a 3′ end of the second nucleic acid strand is annealed to the first nucleic acid strand at an internal position on the first nucleic acid strand.