	US 11,751,591 B2
	*Strain of Oceanobacillus picturae* and applications thereof**
Xiaoyun Xu, Hubei (CN); Ting Wu, Hubei (CN); Jie Song, Hubei (CN); and Siyi Pan, Hubei (CN)
Assigned to Hunzhong Agricultural University, Hubei (CN)
Filed by Huazhong Agricultural University, Hubei (CN)
Filed on Sep. 5, 2020, as Appl. No. 17/13,562.
Claims priority of application No. 202010349212.6 (CN), filed on Apr. 28, 2020.
Prior Publication US 2020/0397027 A1, Dec. 24, 2020
Int. Cl. A23L 19/20 (2016.01); A23L 33/135 (2016.01); A23L 5/20 (2016.01); C12N 1/20 (2006.01); C12R 1/01 (2006.01)

CPC A23L 19/20 (2016.08) [A23L 5/28 (2016.08); A23L 33/135 (2016.08); C12N 1/205 (2021.05); A23V 2002/00 (2013.01); C12R 2001/01 (2021.05)]

1 Claim

1. A freeze-dried strain of Oceanobacillus picturae, which has been deposited in China Center for Type Culture Collection (CCTCC) on Dec. 20, 2019 with a deposit number of CCTCC NO: M 20191070, wherein the strain is prepared by a method of:

coating 1 mL of brine of Xiangyang Pickled Kohlrabi as a sample on a solid medium of high salt LB-Agar powders, wherein every 1000 ml of the solid medium contains 10 g tryptone, 5 g yeast extract, 10 g NaCl, and 18 g agar, culturing at a constant temperature of 37° C., obtaining 10 single colonies by picking and streaking colonies onto corresponding agar plates, inoculating the 10 single colonies into the brine of Xiangyang Pickled Kohlrabi, and screening out the strain according to varieties and contents of volatile substances in the brine.