US 7,589,059 B2
	Delivery of molecules and complexes to mammalian cells in vivo
Jon A. Wolff, Madison, Wis. (US); Vladimir G. Budker, Middleton, Wis. (US); Hans Herweijer, Madison, Wis. (US); James E. Hagstrom, Middleton, Wis. (US); Sean D. Monahan, Madison, Wis. (US); Julia Hegge, Monona, Wis. (US); and Vladimir Subbotin, Madison, Wis. (US)
Assigned to Roche Madison Inc., Madison, Wis. (US)
Filed on Jul. 28, 2003, as Appl. No. 10/628,792.
Claims priority of provisional application 60/398762, filed on Jul. 26, 2002.
Prior Publication US 2004/0023850 A1, Feb. 05, 2004
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 38/00 (2006.01); A61K 38/08 (2006.01); C07K 14/00 (2006.01)

U.S. Cl. 514—2  [514/8; 514/12; 530/300; 530/350]

25 Claims

1. A process for delivering a protein or peptide to an extravascular cell in a mammalian target tissue in vivo comprising: inserting an injection solution containing the protein or peptide into the lumen of an efferent or afferent vessel of the target tissue wherein the inserting of the injection solution results in an increase in pressure in a vein of the target tissue of 10 mm Hg or greater thereby resulting in delivery of the protein or peptide to the extravascular cell.