| US 7,588,936 B2 | ||
| Generating teeth from bone marrow cells | ||
| Paul Thomas Sharpe, London (United Kingdom) | ||
| Assigned to Odontis Ltd., London (United Kingdom) | ||
| Appl. No. 10/546,495 PCT Filed Feb. 17, 2004, PCT No. PCT/GB2004/000635 § 371(c)(1), (2), (4) Date May 23, 2006, PCT Pub. No. WO2004/074464, PCT Pub. Date Sep. 02, 2004. |
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| Claims priority of application No. 0304030.0 (GB), filed on Feb. 21, 2003. | ||
| Prior Publication US 2007/0003529 A1, Jan. 04, 2007 | ||
| Int. Cl. C12N 5/00 (2006.01); C12N 5/06 (2006.01); C12N 5/08 (2006.01) | ||
| U.S. Cl. 435—373 [435/377; 435/371; 435/372] | 1 Claim |
| 1. An in vitro method of generating a tooth progenitor cell, the method comprising the steps of:
a) obtaining human or mouse bone marrow cells comprising mesenchymal stem cells, wherein the bone marrow cells have not undergone
expansion to increase the proportion of a particular cell type present, and
b) incubating the human or mouse bone marrow cells comprising mesenchymal stem cells in the presence of an embryonic oral
epithelial cell for at least twelve hours, wherein when the human or mouse bone marrow cells comprising mesenchymal stem cells
and embryonic oral epithelial cell co-culture is surgically implanted into a subject, the tooth progenitor cell:
i) differentiates into an odontoblast, and
ii) forms a tissue selected from the group consisting of dental pulp, predentin, dentin and combinations thereof.
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