| US 7,588,931 B2 | ||
| High arachidonic acid producing strains of Yarrowia lipolytica | ||
| Howard Glenn Damude, Hockessin, Del. (US); Peter John Gillies, Landenberg, Pa. (US); Daniel Joseph Macool, Philadelphia, Pa. (US); Stephen K. Picataggio, Landenberg, Pa. (US); Dana M. Walters Pollak, Media, Pa. (US); James John Ragghianti, Bear, Del. (US); Zhixiong Xue, Chadds Ford, Pa. (US); Narendra S. Yadav, Chadds Ford, Pa. (US); Hongxiang Zhang, Chadds Ford, Pa. (US); and Quinn Qun Zhu, West Chester, Pa. (US) | ||
| Assigned to E. I. du Pont de Nemours and Company, Wilmington, Del. (US) | ||
| Filed on Nov. 01, 2005, as Appl. No. 11/264,784. | ||
| Claims priority of provisional application 60/624812, filed on Nov. 04, 2004. | ||
| Prior Publication US 2006/0094092 A1, May 04, 2006 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. C12N 1/16 (2006.01); C12N 9/02 (2006.01); C12P 7/64 (2006.01); C07H 21/04 (2006.01) | ||
| U.S. Cl. 435—254.2 [435/254.11; 435/134; 435/189; 536/23.2] | 7 Claims |
| 1. A recombinant oleaginous Yarrowia yeast cell for the production of arachidonic acid comprising at least one gene encoding a Δ8 desaturase wherein;
a) the at least one gene encoding Δ8 desaturase encodes a polypeptide having at least 95% identity to the amino acid sequence
as set forth in SEQ ID NO:49; and
b) the oleaginous Yarrowia yeast cell accumulates in excess of about 25% of its dry cell weight as oil; and
c) the oleaginous Yarrowia yeast cell produces about 14% arachidonic acid of total lipid produced.
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