	US 7,588,891 B2
	Invasive cleavage of nucleic acids
James R. Prudent, Madison, Wis. (US); Jeff G. Hall, Madison, Wis. (US); Victor I. Lyamichev, Madison, Wis. (US); Mary Ann D. Brow, Madison, Wis. (US); and James E. Dahlberg, Madison, Wis. (US)
Assigned to Third Wave Technologies, Inc., Madison, Wis. (US)
Filed on Jul. 15, 2005, as Appl. No. 11/183,077.
Application 09/350309 is a division of application No. 08/756386, filed on Nov. 26, 1996, granted, now 5,985,557.
Application 11/183077 is a continuation of application No. 09/982667, filed on Oct. 18, 2001, granted, now 7,011,944.
Application 09/982667 is a continuation of application No. 09/350309, filed on Jul. 09, 1999, granted, now 6,348,314.
Application 08/756386 is a continuation in part of application No. 08/682853, filed on Jul. 12, 1996, granted, now 6,001,567.
Application 08/682853 is a continuation in part of application No. 08/599491, filed on Jan. 24, 1996, granted, now 5,846,717.
Prior Publication US 2007/0003942 A1, Jan. 04, 2007
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2006.01)

U.S. Cl. 435—6

21 Claims

1. A method of detecting a target nucleic acid, comprising:

a) exposing a sample comprising a target nucleic acid to a purified thermostable FEN-1 endonuclease from an archaebacterial species selected from the group consisting of Pyrococcus furiosus and Methanococcus jannaschii to generate a cleavage product, wherein said cleavage product is produced from a probe oligonucleotide hybridized to said target nucleic acid to form a cleavage structure comprising a duplex and a flap; and

b) detecting said cleavage product, wherein said cleavage product is indicative of the presence of said target nucleic acid.