| US 7,588,885 B2 | ||
| Use of biocistronic DNA constructs for identifying compounds that inhibit IRES-dependent translation | ||
| Tzong-Yuan Wu, Panchiao (Taiwan); Shin-Jhan Zeng, Kaohsiung (Taiwan); Ying-Ju Chen, Shinyuan Township, Pingtung County (Taiwan); Tsu-An Hsu, Taipei (Taiwan); Shin-Ru Shih, Taoyuan (Taiwan); Suey-Sheng Kao, Taipei (Taiwan); and Tzyy-Rong Jinn, Nan Tou (Taiwan) | ||
| Assigned to Chung Yuan Christian University, Jhongli, Taoyuan County (Taiwan) | ||
| Filed on Jul. 23, 2007, as Appl. No. 11/781,596. | ||
| Application 11/781596 is a division of application No. 10/913269, filed on Aug. 06, 2004, granted, now 7,262,051. | ||
| Prior Publication US 2008/0241922 A1, Oct. 02, 2008 | ||
| Int. Cl. C12N 15/63 (2006.01); C12Q 1/68 (2006.01); C12P 21/00 (2006.01) | ||
| U.S. Cl. 435—4 [435/5; 435/7.91; 435/320.1] | 4 Claims |
| 1. A translation regulating system, comprising:
(1) a bicistronic DNA construct containing internal ribosomal entry site (IRES) of encephalomyocarditis virus (EMCV), wherein
said bicistronic DNA construct comprises in sequence, a first reporter gene, which is CAP-dependent initiated, and a second
reporter gene, which is IRES-dependent initiated; and
(2) an amount of amantadine;
wherein said amount of amantadine inhibits the IRES-dependent translation activity in a dose dependent manner without affecting
the CAP-dependent translation activity.
|