| US 7,585,964 B2 | ||
| Methods of analyzing chromosomal translocations using fluorescence in situ hybridization (FISH) | ||
| Nallasivam Palanisamy, North Andover, Mass. (US); and Raju S. Chaganti, New York, N.Y. (US) | ||
| Assigned to Cancer Genetics, Inc., Cambridge, Mass. (US) | ||
| Filed on May 14, 2002, as Appl. No. 10/146,658. | ||
| Claims priority of provisional application 60/357195, filed on Feb. 13, 2002. | ||
| Claims priority of provisional application 60/337653, filed on Nov. 08, 2001. | ||
| Claims priority of provisional application 60/291121, filed on May 14, 2001. | ||
| Prior Publication US 2002/0192692 A1, Dec. 19, 2002 | ||
| Int. Cl. C07H 21/04 (2006.01); C07H 21/02 (2006.01); C07H 21/00 (2006.01); C12Q 1/68 (2006.01); C12P 19/34 (2006.01) | ||
| U.S. Cl. 536—24.3 [536/23.1; 536/24.31; 536/24.33; 536/25.3; 536/25.32; 435/6; 435/91.1] | 11 Claims |
| 1. A set of probes for detecting a human reciprocal chromosomal translocation between a potential breakpoint of a first gene
located on a first human chromosome and a potential breakpoint of a second gene located on a second human chromosome and the
set of probes comprises first and second nucleic acid probes from human genome,
wherein:
(1) the first nucleic acid probe bears a first label and hybridizes to a first region of the first human chromosome and the
first region is outside of the first gene the first region is between the first gene and the centromere of the first human
chromosome, and the first region is within 1 Mb region from the potential breakpoint within the first gene;
(2) the second nucleic acid probe bears a second label and hybridizes to a second region of the human second chromosome and
the second region is outside of the second gene, the second region is between the second gene and one of the telomeres of
the second human chromosome, and the second region is within 1 Mb from the potential breakpoint within the second gene;
(3) the first and second labels are different,
when the first and second human chromosomes have undergone the translocation, the first and second nucleic acid probes hybridize
to a derivative chromosome formed via the translocation between the potential breakpoint of the first gene located on the
first human chromosome and the potential breakpoint of the second gene located on the second human chromosome, such that both
the first and second labels appear on the derivative chromosome; and
(ii) when the first and second chromosomes have not undergone the translocation, the first and second nucleic acid probes
hybridize to the first and second human chromosomes respectively, such that only the first label appears on the first human
chromosome and only the second label appears on the second human chromosome;
and wherein:
(a) the first gene is human BCL1 gene (BCL1), the second gene is human IGH gene (IGH), the first human chromosome is human
chromosome 11, the second human chromosome is human chromosome 14, and the human reciprocal chromosomal translocation is t(11;14)(q13;q32);
or
(b) the first gene is human IGH gene, the second gene is human BCL-2 gene (BCL2), the first human chromosome is human chromosome
14, the second chromosome is human chromosome 18, and the human reciprocal chromosomal translocation is t(14;18)(q32;q21);
or
(c) the first gene is human MYC gene (MYC), the second gene is human IGH gene, the first human chromosome is human chromosome
8, the second human chromosome is human chromosome 14, and the human reciprocal chromosomal translocation is t(8;14)(q24;q32);
or
(d) the first gene is human BCR gene (BCR), the second gene is human ABL gene (ABL), the first human chromosome is human chromosome
22, the second human chromosome is human chromosome 9, and the human reciprocal chromosomal translocation is t(9;22)(q34;q11),
or
(e) the first gene is human IGH gene (IGH), the second gene is human BCL1 gene (BCL1), the first human chromosome is human
chromosome 14, the second human chromosome is human chromosome 11, and the human reciprocal chromosomal translocation is t(11;14)(q13;q32);
or
(f) the first gene is human BCL-2 gene (BCL2), the second gene is human IGH gene, the first human chromosome is chromosome
18, the second human chromosome is human chromosome 14 and the reciprocal human chromosomal translocation is t(14;18)(q32;q21);
or
(g) the first gene is human IGH gene, the second gene is human MYC gene (MYC), the first human chromosome is human chromosome
14, the second human chromosome is human chromosome 8 and the human reciprocal chromosomal translocation is t(8;14)(q24;q32);
or
(h) the first gene is human ABL gene (ABL), the second gene is human BCR gene (BCR), the first human chromosome is human chromosome
9, the second human chromosome is human chromosome 22, and the human reciprocal chromosomal translocation is t(9;22)(q34;q11).
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