| US 7,585,644 B2 | ||
| Mycopolasma detecting methods and materials | ||
| Kevin John Slater, Nottingham (United Kingdom); Anthony Pitt, Nottingham (United Kingdom); Anne Cox, Nottingham (United Kingdom); and Sharon Patricia Mary Crouch, Nottingham (United Kingdom) | ||
| Assigned to Cambrex Bio Science Nottingham, Ltd., Nottingham (United Kingdom) | ||
| Filed on Apr. 16, 2004, as Appl. No. 10/825,607. | ||
| Claims priority of provisional application 60/463323, filed on Apr. 17, 2003. | ||
| Claims priority of application No. 0308829.1 (GB), filed on Apr. 17, 2003. | ||
| Prior Publication US 2004/0265942 A1, Dec. 30, 2004 | ||
| Int. Cl. C12Q 1/50 (2006.01) | ||
| U.S. Cl. 435—17 | 45 Claims |
| 1. A method of detecting the presence of potential contaminating mycoplasma in a test sample not known to contain mycoplasma
comprising:
(i) providing the test sample;
(ii) detecting and/or measuring the activity of an enzyme selected from the group consisting of acetate kinase, carbamate
kinase, and a mixture thereof in the test sample, and said activity being indicative of the presence of potential contaminating
mycoplasma; and
(iii) identifying the test sample as potentially contaminated with mycoplasma on the basis of detection and/or measurement
of said activity in step (ii).
|