| US 7,585,621 B2 | ||
| Detection of West Nile virus infection and vaccination | ||
| Melissa Beall, Cape Elizabeth, Me. (US); and Ramaswamy Chandrashekar, Scarborough, Me. (US) | ||
| Assigned to IDEXX Laboratories, Inc., Westbrook, Me. (US) | ||
| Filed on Sep. 09, 2004, as Appl. No. 10/937,157. | ||
| Claims priority of provisional application 60/501390, filed on Sep. 09, 2003. | ||
| Prior Publication US 2005/0053923 A1, Mar. 10, 2005 | ||
| Int. Cl. C12Q 1/70 (2006.01); A61K 39/12 (2006.01) | ||
| U.S. Cl. 435—5 [424/218.1] | 8 Claims |
| 1. A method for determining whether an animal is infected with West Nile Virus (WNV), or is either not infected or is vaccinated
with a WNV subunit vaccine, the method comprising:
(a) contacting a biological sample from the animal with a first WNV polypeptide that is not an element of the WNV subunit
vaccine, wherein the first WNV polypeptide comprises SEQ ID NO:2, and wherein the sample comprises at least one antibody;
(b) contacting the biological sample with a second WNV polypeptide that is an element of the WNV subunit vaccine; and
(c) detecting whether the first and the second WNV polypeptides substantially bind to an antibody in the sample,
wherein if an antibody in the sample substantially binds to the first WNV polypeptide, the animal is naturally infected with
WNV, if an antibody in the sample substantially binds to the second WNV polypeptide but not the first WNV polypeptide, the
animal has been vaccinated but is not naturally infected, and if an antibody in the sample does not substantially bind to
either the first or the second WNV polypeptide, the animal is not vaccinated and not infected.
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