a) anesthetizing said eye with at least one of a topical anesthetic, retro bulbar anesthesia, and peribulbar anesthesia;

b) securing the eyelids in a spaced-apart relationship using an eyelid speculum;

c) providing additional magnification of a portion of the iris to be colored;

d) constricting the pupil of said eye using a pre-operative miotic or an intra-operative miotic;

e) making first and second incisions into the anterior chamber of said eye on opposite sides of the cornea of said eye;

f) removing aqueous residue from the angle of said anterior chamber proximate the edge of said pupil using a cannula, while avoiding damage to other interocular structures;

g) drying a portion of said iris of said eye by concurrently infusing a blowing gas through said first incision and aspirating said blowing gas through said second incision at a rate that maintains inflation of said eye but does not over-inflate said eye;

h) applying colorant to said iris of said eye;

i) drying said applied colorant;

j) exchanging said blowing gas with balanced salt solution;

k) providing interocular pressure in the physiologic range;

l) applying at least one of antibiotic and anti-inflammatory drops to said eye; and

m) removing said eyelid speculum.