	US 11,739,442 B2
	TCR libraries
Bent Karsten Jakobsen, Abingdon (GB); Nathaniel Ross Liddy, Abingdon (GB); Peter Eamon Molloy, Abingdon (GB); and Annelise Brigitte Vuidepot, Abingdon (GB)
Assigned to Immunocore Limited, Abingdon (GB); and Adaptimune Limited, Abingdon (GB)
Filed by ADAPTIMMUNE LIMITED, Abingdon (GB); and IMMUNOCORE LIMITED, Abingdon (GB)
Filed on Aug. 19, 2021, as Appl. No. 17/406,954.
Application 17/406,954 is a continuation of application No. 15/759,775, granted, now 11,124,556, previously published as PCT/EP2016/071757, filed on Sep. 15, 2016.
Claims priority of application No. 1516272 (GB), filed on Sep. 15, 2015.
Prior Publication US 2021/0395332 A1, Dec. 23, 2021
Int. Cl. C40B 30/04 (2006.01); C07K 14/725 (2006.01); C12N 15/10 (2006.01); C40B 40/02 (2006.01); C40B 40/10 (2006.01)

CPC C40B 30/04 (2013.01) [C07K 14/7051 (2013.01); C12N 15/1037 (2013.01); C40B 40/02 (2013.01); C40B 40/10 (2013.01); C07K 2317/565 (2013.01)]

4 Claims

1. A method of obtaining a T cell receptor that specifically binds a peptide antigen, comprising screening a library of particles displaying a plurality of different T cell receptors (TCRs) with the peptide antigen, wherein the plurality of TCRS consists essentially of TCRs comprising (a) an alpha chain variable domain (Vα) that is a TRAV12-2 gene product from a natural repertoire of TCRs, and (b) a beta chain, the beta chain comprising a beta chain variable domain (Vβ) that is a gene product of a TRBV gene, wherein the Vα CDR3 length varies among the plurality of displayed TCRs and the Vβ CDR3 length varies among the plurality of displayed TCR, wherein the TCRs are covalently linked to the particles, and wherein the particles are phage particles, ribosomes, yeast cells, or mammalian cells, the method comprising:

(1) panning the library using as a target the peptide antigen;

(2) repeating step (1) one or more times;

(3) screening the phage clones identified in step (1) or (2); and

(4) identifying a TCR that specifically binds the peptide antigen.