CPC C12Q 1/6869 (2013.01) [C07K 14/35 (2013.01)] | 15 Claims |
1. A method of characterizing a target polynucleotide, comprising:
a) providing a transmembrane MspA pore and a polynucleotide binding protein in which a part of the transmembrane MspA pore which interacts with the polynucleotide binding protein and a part of the polynucleotide binding protein which interacts with the transmembrane MspA pore has been modified,
wherein the modification of the transmembrane MspA pore comprises an amino acid substitution, insertion, or deletion relative to an unmodified transmembrane MspA pore, and wherein the modification of the polynucleotide binding protein comprises an amino acid substitution, insertion, or deletion relative to an unmodified polynucleotide binding protein;
b) contacting the transmembrane MspA pore and polynucleotide binding protein provided in (a) with the target polynucleotide such that the polynucleotide binding protein controls the movement of the target polynucleotide with respect to the transmembrane MspA pore; and
c) taking one or more electrical or optical measurements as the target polynucleotide moves with respect to the transmembrane MspA pore.
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